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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">potato</journal-id><journal-title-group><journal-title xml:lang="ru">Картофелеводство</journal-title><trans-title-group xml:lang="en"><trans-title>Potato Growing</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0134-9740</issn><publisher><publisher-name>Научно-практический центр Национальной академии наук Беларуси по картофелеводству и плодоовощеводству</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">potato-206</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>БИОТЕХНОЛОГИЧЕСКИЕ МЕТОДЫ В СЕЛЕКЦИИ И СЕМЕНОВОДСТВЕ КАРТОФЕЛЯ</subject></subj-group></article-categories><title-group><article-title>ПРОТЕОМНЫЙ АНАЛИЗ ТРАНСГЕННЫХ РАСТЕНИЙ КАРТОФЕЛЯ, ЭКСПРЕССИРУЮЩИХ ГЕНЫ АНТИМИКРОБНЫХ ПЕПТИДОВ, ДЛЯ ОЦЕНКИ ИХ ФУНКЦИОНАЛЬНОГО СОСТОЯНИЯ</article-title><trans-title-group xml:lang="en"><trans-title>PROTEOMICANALYSIS OFTRANSGENIC POTATO PLANTS EXPRESSING THE GENES OFANTIMICROBIALPEPTIDES FOR EVALUATION OFTHEIR FUNCTIONAL STATUS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бакакина</surname><given-names>Ю. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Bakakina</surname><given-names>Yu. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>г. Минск</p></bio><email xlink:type="simple">bakakinay@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гапеева</surname><given-names>Т. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Gapeeva</surname><given-names>T. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>г. Минск</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дубовская</surname><given-names>Л. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Dubovskaya</surname><given-names>L. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>г. Минск</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Волотовский</surname><given-names>И. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Volotovskiy</surname><given-names>I. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>г. Минск</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>ГНУ «Институт биофизики и клеточной инженерии НАН Беларуси»</institution><country>Belarus</country></aff><pub-date pub-type="collection"><year>2015</year></pub-date><pub-date pub-type="epub"><day>28</day><month>04</month><year>2022</year></pub-date><volume>23</volume><issue>1</issue><fpage>69</fpage><lpage>77</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Бакакина Ю.С., Гапеева Т.А., Дубовская Л.В., Волотовский И.Д., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Бакакина Ю.С., Гапеева Т.А., Дубовская Л.В., Волотовский И.Д.</copyright-holder><copyright-holder xml:lang="en">Bakakina Y.S., Gapeeva T.A., Dubovskaya L.V., Volotovskiy I.D.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://potato.belal.by/jour/article/view/206">https://potato.belal.by/jour/article/view/206</self-uri><abstract><p>Проведена оценка протеомного статуса трансгенных линий растений картофеля с экспрессируемыми генами антимикробных пептидов цекропин-мелиттинового типа, полученных на основе сортов белорусской селекции. Показано, что в клубнях трансгенных линий наблюдается повышенное накопление белков семейства пататинов, что может быть следствием активации защитной системы растений под воздействием конститутивного трансгенного синтеза антимикробных пептидов.</p></abstract><trans-abstract xml:lang="en"><p>The proteomic evaluation of transgenic potato plants derived from the cultivars of Belarusian breeding expressing the genes of antimicrobial peptides of the cecropin-melittin type was conducted. The increased accumulation of patatin proteins was observed. That fact can be a result of plant defense system activation under constitutive transgenic synthesis of antimicrobial peptides.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>антимикробные пептиды</kwd><kwd>защита растений</kwd><kwd>пататины</kwd><kwd>протеомный анализ</kwd><kwd>трансгенные растения Solanum tuberosum</kwd></kwd-group><kwd-group xml:lang="en"><kwd>antimicrobial peptides</kwd><kwd>transgenic plants of Solanum tuberosum</kwd><kwd>patatins</kwd><kwd>proteomic analysis</kwd><kwd>transgenic plants Solanum tuberosum</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Бурьянов, Я.И. Стратегия создания трансгенных растений с устойчивостью к фитопатогенам и вредителям / Я.И. Бурьянов, К.И. Кадо // Биоорганич. химия. – 1999. – Т. 25. – № 12. – С. 903–910.</mixed-citation><mixed-citation xml:lang="en">Бурьянов, Я.И. Стратегия создания трансгенных растений с устойчивостью к фитопатогенам и вредителям / Я.И. Бурьянов, К.И. Кадо // Биоорганич. химия. – 1999. – Т. 25. – № 12. – С. 903–910.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Трансгенные растения картофеля белорусских сортов, экспрессирующие гены антимикробных пептидов цекропин-мелиттинового типа / Н.Л. Вутто [и др.] // Генетика. – 2010. – Т. 46. – № 9. – С.1–9.</mixed-citation><mixed-citation xml:lang="en">Трансгенные растения картофеля белорусских сортов, экспрессирующие гены антимикробных пептидов цекропин-мелиттинового типа / Н.Л. Вутто [и др.] // Генетика. – 2010. – Т. 46. – № 9. – С.1–9.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Оценка устойчивости трансгенных растений картофеля белорусских сортов с генами антимикробных пептидов цекропин-мелиттинового типа к заражению Phytophthora infestans / Н.Л. Вутто [и др.] // Вес. Нац. акад. навук Беларусi. Сер. бiял. навук. – 2012. – № 2. – С. 69–73.</mixed-citation><mixed-citation xml:lang="en">Оценка устойчивости трансгенных растений картофеля белорусских сортов с генами антимикробных пептидов цекропин-мелиттинового типа к заражению Phytophthora infestans / Н.Л. Вутто [и др.] // Вес. Нац. акад. навук Беларусi. Сер. бiял. навук. – 2012. – № 2. – С. 69–73.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Bradford, M.M. A rapid and sensitive method for the quantitation of microgramquantities of protein utilizingthe principle of protein-dye binding / M.M. Bradford // Anal. Biochem. – 1976. – Vol. 72. – P. 248–254.</mixed-citation><mixed-citation xml:lang="en">Bradford, M.M. A rapid and sensitive method for the quantitation of microgramquantities of protein utilizingthe principle of protein-dye binding / M.M. Bradford // Anal. Biochem. – 1976. – Vol. 72. – P. 248–254.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Пальцев, М.А. Молекулярная медицина и прогресс фундаментальных наук / М.А. Пальцев // Вестник Российской акад. наук. – 2002. – Том 72. – № 1. – C. 13–21.</mixed-citation><mixed-citation xml:lang="en">Пальцев, М.А. Молекулярная медицина и прогресс фундаментальных наук / М.А. Пальцев // Вестник Российской акад. наук. – 2002. – Том 72. – № 1. – C. 13–21.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">The effect of storage of whole potatoes of three cultivars on the patatin and protease inhibitor content; a study using capillary electrophoresis and MALDI-TOF mass spectrometry / A.M. Pots [et al.] // J Science of Food and Agricult. – 1999. – Vol. 79. – P. 1557–1564.</mixed-citation><mixed-citation xml:lang="en">The effect of storage of whole potatoes of three cultivars on the patatin and protease inhibitor content; a study using capillary electrophoresis and MALDI-TOF mass spectrometry / A.M. Pots [et al.] // J Science of Food and Agricult. – 1999. – Vol. 79. – P. 1557–1564.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Gene expression during tuber development in potato plants / S. Prat [et al.] // FEBS Letters. – 1990. – Vol. 268. – P. 334–338.</mixed-citation><mixed-citation xml:lang="en">Gene expression during tuber development in potato plants / S. Prat [et al.] // FEBS Letters. – 1990. – Vol. 268. – P. 334–338.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Gene expression during tuber development in potato plants / S. Prat [et al.] // FEBS Letters. – 1990. – Vol. 268. – P. 334–338.</mixed-citation><mixed-citation xml:lang="en">Gene expression during tuber development in potato plants / S. Prat [et al.] // FEBS Letters. – 1990. – Vol. 268. – P. 334–338.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Purification and characterization of s–xylosidase from potatoes (Solanum tuberosum) / C. Peyer [et al.] // BBA-Proteins and Proteomics. – 2004. – Vol. 1672. – P. 27–35.</mixed-citation><mixed-citation xml:lang="en">Purification and characterization of s–xylosidase from potatoes (Solanum tuberosum) / C. Peyer [et al.] // BBA-Proteins and Proteomics. – 2004. – Vol. 1672. – P. 27–35.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Barta, J. Patatin, the major protein of potato (Solanum tuberosum L.) Tubers, and its occurrence as genotype effect: processing versus table potatoes / J. Barta, V. Bartova // Czech J. Food Sci. – Vol. 26. – № 5. – P. 347–359.</mixed-citation><mixed-citation xml:lang="en">Barta, J. Patatin, the major protein of potato (Solanum tuberosum L.) Tubers, and its occurrence as genotype effect: processing versus table potatoes / J. Barta, V. Bartova // Czech J. Food Sci. – Vol. 26. – № 5. – P. 347–359.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">A pathogen-inducible patatin-like lipid acyl hydrolase facilitates fungal and bacterial host colonization in Arabidopsis / S. La Camera [et al.] // Plant J. – 2005. – Vol. 44. – № 5. – P. 810–825.</mixed-citation><mixed-citation xml:lang="en">A pathogen-inducible patatin-like lipid acyl hydrolase facilitates fungal and bacterial host colonization in Arabidopsis / S. La Camera [et al.] // Plant J. – 2005. – Vol. 44. – № 5. – P. 810–825.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Production of cecropin A in transgenic rice plants has an impact on host gene expression / S. Campo [et al.] // Plant Biotechnol. J. – 2008. – Vol. 6. – P. 585–608.</mixed-citation><mixed-citation xml:lang="en">Production of cecropin A in transgenic rice plants has an impact on host gene expression / S. Campo [et al.] // Plant Biotechnol. J. – 2008. – Vol. 6. – P. 585–608.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
