potatoКартофелеводствоPotato Growing0134-9740Научно-практический центр Национальной академии наук Беларуси по картофелеводству и плодоовощеводству10.47612/0134-9740-2022-30-46-54potato-309Research ArticleГЕНЕТИКА КАРТОФЕЛЯPOTATO GENETICSПЦР-праймеры и зонды для оценки экспрессии генов изоформ фосфолипазы С в клетках трансгенных растений картофеля с антисмысловой конструкцией для гена фосфолипазы СPCR primers and probes for evaluating the expression of phospholipase C isoform genes in transgenic cells of potato plants with antisense construction for phospholipase C geneГапееваТ. А.GapeevaT. A.

г. Минск

gapeeva@lab.ibp.org.byПундикА. Н.PundikA. N.

г. Минск

СеманюкТ. В.SemanyukT. V.

аг. Самохваловичи, Минский район

ГНУ«Институт биофизики и клеточной инженерии Национальной академии наук Беларуси»BelarusООО «ГлобалГен»BelarusРУП «Научно-практический центр Национальной академии наук Беларуси по картофелеводству и плодоовощеводству»Russian Federation2022020320233014654Copyright © Гапеева Т.А., Пундик А.Н., Семанюк Т.В., 20232023Гапеева Т.А., Пундик А.Н., Семанюк Т.В.Gapeeva T.A., Pundik A.N., Semanyuk T.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://potato.belal.by/jour/article/view/309

Разработаны ПЦР-праймеры, а также «TaqMan»-наборы для оценки экспрессии генов изоформ фосфолипазы C (ФлС) картофеля дикого типа (WT) и трансгенных растений, экспрессирующих антисмысловую РНК для ингибирования генов ФлС. Показано, что использование «TaqMan»-наборов для генов изоформ ФлС1 и ФлС3, а также гена-нормализатора ef1a в дуплексной ПЦР в реальном времени позволяет определять дифференциальную экспрессию соответствующих изоформ в различных тканях растений картофеля. Методом ПЦР с детекцией в «конечной точке» показано уменьшение экспресcии генов изоформ ФлС в клетках трансгенных растений по сравнению с WT-картофелем.

PCR primers, as well as TaqMan-kits have been developed for evaluating gene expression of phospholipase C (PLC) isoforms in wild-type potato (WT) and transgenic plants expressing antisense RNA to inhibit PLC genes. Using the TaqMan- kits for the genes of PLC1 and PLC3 isoforms, as well as for the gene-normalizer ef1ain duplex realtime PCR determines the differential gene expression of the PLC isoforms in various tissues of potato plants. The «endpoint» PCR established a decrease in the expression of the PLC genes in transgenic plants compared with WT

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The authors declare that there are no conflicts of interest present.