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CREATION OFTRANSGENIC POTATO PLANTS WITH GENE OFYEASTALA-SYNTHETASES

Abstract

The binary vector system with the gene for the synthesis of ALA-synthetases of Saccharomyces cerevisiae in cytoplasm of plant cell was constructed. Using this vector system the transgenic plants of potato of Belarussian variety Scarb were obtained. The plants will be applied for the development of transgenic potato cultivars synthesizing ALA and possessing increasing resistance to unfavorable environmental conditions.

About the Authors

T. A. Gapeeva
ГНУ «Институт биофизики и клеточной инженерии Национальной академии наук Беларуси»
Belarus


T. G. Tretyakova
ГНУ «Институт биофизики и клеточной инженерии Национальной академии наук Беларуси»
Belarus


A. V. Zaitseva
ГНУ «Институт биофизики и клеточной инженерии Национальной академии наук Беларуси»
Belarus


I. D. Volotovskij
ГНУ «Институт биофизики и клеточной инженерии Национальной академии наук Беларуси»
Belarus


References

1. Яронская, Е.Б. Экологически безопасные регуляторы роста растений на основе 5-аминолевулиновой кислоты / Е.Б. Яронская, Н.Г. Аверина, М.А. Кисель / Труды БГУ. – 2012. – Т. 7. – Ч. 1. – С.127–134.

2. Wang, L.J. Promotion of 5-aminolevulinic acid on photosynthesis of melon (Cucumis melo) seedlings under low light and chilling stress conditions / L.J. Wang, W.B. Jiang, B.J. Huang // Physiol. Plant. – 2004. – V. 121. – № 2. – Р. 258–264.

3. Improvement of cold resistance in rice seedlings by 5-aminolevulinic acid / Y. Hotta [et al.] // J. Pesticide Sci. – 1998. – V. 23. – P. 29–33.

4. The role of 5-aminolevulinic acid in the response to cold stress in soybean plants / K.B. Balestrasse [et al.] // Phytochemistry. – 2010. – V. 71. – P. 2038–2045.

5. Korkmaz, A. Promotion by 5-aminolevulinic acid of pepper seed germination and seedling emergence under low temperature stress /A. Korkmaz, Y Korkmaz // Sci Hortic. – 2009. – V. 119. – P. 98–102.

6. Effect of 5-аminolevulinic аcid (ALA) on Leaf Diurnal Photosynthetic Characteristics and Antioxidant Activity in Pear (Pyrus Pyrifolia Nakai) / M. Shen, Z.P. Zhang, L.J. Wang // Artificial Photosynthesis. – 2012. – V. 11. – P. 240–256.

7. 5-аminolevulinic acid pretreatment mitigates drought stress of cucumber leaves through altering antioxidant enzyme activity / D-M. Li [et al.] // Scientia Horticulturae. – 2011. – V. 130, I. 4. – P. 820–828.

8. Role of 5-aminolevulinic acid (ALA) on active oxygen-scavenging system in NaCl-treated spinach (Spinacia oleracea) / E. Nishihara [et al.] // J. Plant Physiol. – 2003. – V. 160. – P. 1085–1091.

9. Lazo, G.R. DNA transformation-competent Arabidopsis genomic library in Agrobacterium / G.R. Lazo [et al.] // Biotechnology. – 1991. – V. 9. – P. 963–967.

10. Hood, E.E. The Hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA / E.E Hood [et al.] // J. Bact. – 1986. – V. 168. – P. 1291–1304.

11. Харди, К. Выделение бактериальных плазмид / К. Харди // Плазмиды. Методы / П. Бергквист [и др.]; под ред. К. Харди. – М., 1990. – Гл. 1. – С. 11–18.

12. Sambrook, J. W. Molecular cloning: A Laboratory manual / J. W. Sambrook, E. F. Fritsch, T. Maniatis. – N.Y: Cold Spring Harb. Lab.Press, 1989. – Vol. 1, 2, 3.

13. Ханаан, Д. Методы трансформации E. сoli / Д. Ханаан // Клонирование ДНК. Методы/ С. Бэлдэри [и др.]; под ред. Д.М. Гловера . – М., 1988. – Гл. 6. – С. 140–173.

14. Draper, J. Transformation of dicotyledonous plant cells using Ti plasmid of Agrobacterium tumefaciens and Ri plasmid of A.rhizogenes / J. Draper [et al.] // Plant Genetic transformation and Gene expression: A Laboratory Manual / Eds. J. Draper [et al.]. – Oxford, 1988. – Chap. 2. – P. 69–160.

15. Murashige, T. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures / T. Murashige [et al.] // Physiol. Plant. – 1962. – Vol. 15. – P. 473–497.

16. Kеng, T. The NineAmino-Terminal Residues of 8-Aminolevulinate Synthase Direct 1-Galactosidase into the Mitochondrial Matrix / T. Kеng [et al.] // 1986. – Mol. Cell. Biol. – V. 6. – № 2. – P. 355–364.

17. Urban-Grimal, D. The nucleotide sequence of the HEM1 gene and evidence for a precursor form of the mitochondrial 5-aminolevulinate synthase in Saccharomyces cerevisiae / D. Urban-Grimal [et al.] // Eur. J. Biochem. – 1986. – V. 156. – P. 511–519.


Review

For citations:


Gapeeva T.A., Tretyakova T.G., Zaitseva A.V., Volotovskij I.D. CREATION OFTRANSGENIC POTATO PLANTS WITH GENE OFYEASTALA-SYNTHETASES. Potato Growing. 2014;22(1):50-57. (In Russ.)

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ISSN 0134-9740 (Print)