PCR primers and probes for evaluating the expression of phospholipase C isoform genes in transgenic cells of potato plants with antisense construction for phospholipase C gene
https://doi.org/10.47612/0134-9740-2022-30-46-54
Abstract
PCR primers, as well as TaqMan-kits have been developed for evaluating gene expression of phospholipase C (PLC) isoforms in wild-type potato (WT) and transgenic plants expressing antisense RNA to inhibit PLC genes. Using the TaqMan- kits for the genes of PLC1 and PLC3 isoforms, as well as for the gene-normalizer ef1ain duplex realtime PCR determines the differential gene expression of the PLC isoforms in various tissues of potato plants. The «endpoint» PCR established a decrease in the expression of the PLC genes in transgenic plants compared with WT
About the Authors
T. A. Gapeeva
ГНУ«Институт биофизики и клеточной инженерии Национальной академии наук Беларуси»
Belarus
Belarus
A. N. Pundik
ООО «ГлобалГен»
Belarus
Belarus
T. V. Semanyuk
РУП «Научно-практический центр Национальной академии наук Беларуси по картофелеводству и плодоовощеводству»
Russian Federation
Russian Federation
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Review
For citations:
Gapeeva T.A., Pundik A.N., Semanyuk T.V. PCR primers and probes for evaluating the expression of phospholipase C isoform genes in transgenic cells of potato plants with antisense construction for phospholipase C gene. Potato Growing. 2022;30(1):46-54. (In Russ.) https://doi.org/10.47612/0134-9740-2022-30-46-54
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