POTATOES PLANTS SOLANUM TUBEROSUM EXPRESSING THE GENE D12-ACYL-LIPIDIC DESATURASE OFCYANOBACTERIA
Abstract
In the conditions of climatic changes plants suffer from temperature fluctuations. It is possible to reduce temperature of transition from a gel phase to liquid crystal thanks to changes of fat and acid structure of membrane lipids towards increase in the share of nonsaturated fatty acids. Desaturase are enzymes which promote formation of double communications in fatty acids. The desA gene coding the D12-acyl-lipidic desaturase of a cyanobacterium of Synechocystis sp. PCC 6803 is used in the work. Plants of Solanum tuberosum potatoes of the Ukrainian varities which expressirut this gene are received. In these plants the insert of hybrid genes and an expression of reporterny white were proved. At transgenes investigated a range of fatty acids by method of gas chromatography and mass spectrometry. It is found out that plants which expressirut desA gene differ in the increased content of linoleic acid in comparison with control.
About the Authors
T. M. Kirpa-Nesmiyan
Институт клеточной биологии и генетической инженерии НАН Украины
Ukraine
Ukraine
V. А. Rudas
Институт клеточной биологии и генетической инженерии НАН Украины
Ukraine
Ukraine
V. A. Osipenko
Национальный технический университет Украины «Киевский политехнический институт»
Ukraine
Ukraine
M. A. Kharhota
Институт микробиологии и вирусологии им. Д.К. Заболотного НАН Украины
Ukraine
Ukraine
N. V. Kuchuk
Институт клеточной биологии и генетической инженерии НАН Украины
Ukraine
Ukraine
References
1. Los, D.A. Membrane fluidity and its roles in the perception of environmental signals / D.A. Los, N. Murata // Biochim Biophys Acta. – 2004. – V. 1666. – Р. 142–157.
2. Murashige, T. A revised medium for rapid growth and bioassays with tobacco tissue culture / T. Murashige, F. Skoog // Physiol. Plant. – 1962. – V. 15, № 3. – P. 473–497.
3. Querci, M. The analysis of food samples for the presence of genetically modified organisms / M. Querci, M. Jermini, G. Van der Fede // Luxembourg: Office for Official Publications of the European Communities. – 2006 – Р. 229.
4. Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants / I.N. Berdichevets [et al.] // Anal. Bioanal. Chem. – 2010. – V. 397. – P. 2289–2293.
5. A reporter system for prokaryotic and eukaryotic cells based on the thermostable lichenase from Clostridium thermocellum / E. Piruzian [et al.] // Mol. Genet. Genom. – 2002. – V. 266. – P. 778–786.
6. Garces, R. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues / R. Garces, M. Mancha // Analytical Biochemestry. – 1993. – V. 211. – P. 139–143.
Review
For citations:
Kirpa-Nesmiyan T.M., Rudas V.А., Osipenko V.A., Kharhota M.A., Kuchuk N.V. POTATOES PLANTS SOLANUM TUBEROSUM EXPRESSING THE GENE D12-ACYL-LIPIDIC DESATURASE OFCYANOBACTERIA. Potato Growing. 2016;24(1):81-87. (In Russ.)
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