PROTEOLYTIC ENZYMES SECRETED BY TWO ISOLATES FUNGUS ALTERNARIA SOLANI SORAUER
Abstract
Serine proteinases secretion by two genetically related isolates of the fungus, collected from potato and tomato plants grown in central Russia have been studied. The data clarify functions of these enzymes in the process of pathogenesis in which they can play a pivotal role. It was found that the two isolates produced trypsin-like and subtilisin-like proteinases during growth both in synthetic culture medium and in medium containing heat-stable vegetable proteins. There were significant differences in the influence of the environment on the serine proteinase secretion by the potato and tomato isolates of A. solani. The proportion of such serine proteinases as trypsin-like and subtilisin-like enzymes depends on the composition of the growth medium, especially on the available organic nitrogen form, as well as features both of the pathogenic fungus and of the host plant. So, the tomato isolate demonstrated weak growth and low level or absence of serine proteinase excretion on cultivation with the medium containing proteins extracted from potato tubers and pea seeds. The potato isolate secreted many more serine proteinases, among which the trypsinlike enzymes dominated. Our data suggest that the tomato isolate, when grown on medium with proteins extracted from potato tubers, lost pathogenicity and became to behave as a saprophyte, while the potato isolate retained its pathogenic properties on growth on any tested medium.
About the Authors
T. A. Valueva
Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской Академии Наук
Russian Federation
Russian Federation
N. N. Kudryavtseva
Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской Академии Наук
Russian Federation
Russian Federation
E. L. Gvozdeva
Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской Академии Наук
Russian Federation
Russian Federation
A. V. Sof'in
Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской Академии Наук
Russian Federation
Russian Federation
M. A. Pobedinskaya
Кафедра микологии, Московский государственный университет им. М.В. Ломоносова
Russian Federation
Russian Federation
S. Elansky
Кафедра микологии, Московский государственный университет им. М.В. Ломоносова
Russian Federation
Russian Federation
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Review
For citations:
Valueva T.A., Kudryavtseva N.N., Gvozdeva E.L., Sof'in A.V., Pobedinskaya M.A., Elansky S. PROTEOLYTIC ENZYMES SECRETED BY TWO ISOLATES FUNGUS ALTERNARIA SOLANI SORAUER. Potato Growing. 2013;21(1):234-246. (In Russ.)
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